Sokoine University of Agriculture

Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania

Show simple item record Mbugi, Erasto V Mutayoba, Benezeth M Malisa, Allen L Balthazary, Sakurani T Nyambo, Thomas B Mshinda, Hassan 2016-11-11T07:30:38Z 2016-11-11T07:30:38Z 2006-10
dc.description Malaria Journal en_US
dc.description.abstract Background: Sulphadoxine-pyrimethamine (SP) has been and is currently used for treatment of uncomplicated Plasmodium falciparum malaria in many African countries. Nevertheless, the response of parasites to SP treatment has shown significant variation between individuals. Methods: The genes for dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) were used as markers, to investigate parasite resistance to SP in 141 children aged less than 5 years. Parasite DNA was extracted by Chelex method from blood samples collected and preserved on filter papers. Subsequently, polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were applied to detect the SP resistance-associated point mutations on dhfr and dhps. Commonly reported point mutations at codons 51, 59, 108 and 164 in the dhfr and codons 437, 540 and 581 in the dhps domains were examined. Results: Children infected with parasites harbouring a range of single to quintuple dhfr/dhps mutations were erratically cured with SP. However, the quintuple dhfr/dhps mutant genotypes were mostly associated with treatment failures. High proportion of SP resistance-associated point mutations was detected in this study but the adequate clinical response (89.4%) observed clinically at day 14 of follow up reflects the role of semi-immunity protection and parasite clearance in the population. Conclusion: In monitoring drug resistance to SP, concurrent studies on possible confounding factors pertaining to development of resistance in falciparum malaria should be considered. The SP resistance potential detected in this study, cautions on its useful therapeutic life as an interim firstline drug against malaria in Tanzania and other malaria-endemic countries. en_US
dc.language.iso en en_US
dc.publisher Malaria Journal en_US
dc.subject Sulphadoxine-pyrimethamine en_US
dc.subject Plasmodium falciparum en_US
dc.subject Drug resistance en_US
dc.subject Plasmodium falciparum malaria en_US
dc.subject Mlimba, Tanzania en_US
dc.title Drug resistance to sulphadoxine-pyrimethamine in Plasmodium falciparum malaria in Mlimba, Tanzania en_US
dc.type Article en_US
dc.url en_US

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