Sokoine University of Agriculture

Polymorphisms of BoLA-DRB 3.2 gene and associated genetic relationships among four strains of Tanzania shorthorn zebu cattle

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dc.contributor.author Lubambe, Dominick
dc.contributor.author Msalya, George
dc.contributor.author Kipanyula, Maulilio
dc.contributor.author Karimuribo, Esron
dc.contributor.author Chenyambuga, Sebastian
dc.date.accessioned 2021-06-30T06:55:39Z
dc.date.available 2021-06-30T06:55:39Z
dc.date.issued 2018
dc.identifier.issn 2586-4297
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/3626
dc.description Journal Article en_US
dc.description.abstract Bovine Lymphocyte Antigen (BoLA) genes play important roles in resistance or susceptibility of cattle to infectious diseases. The BoLA gene comprises of several loci including the most polymorphic site namely DRB 3.2. We amplified 200 DNA samples and sequenced 270 bp comprising exon 2 of BoLA-DRB 3.2 in four strains of Tanzania shorthorn zebu (TSZ) cattle (Tarime, Sukuma, Maasai, Singida white) and one breed, namely Friesian. Sequences were processed on Finch TV, aligned on Basic Local Alignment Search Tool (BLAST) online, and matched to amino acids using MEGA 6. Frequency of each allele was computed as proportion of total alleles in each population. Chi-square was used to test significance in allele frequencies. Heterozygosities were computed using Poptree 2. Putative evolutionary relationships were evaluated by Nei genetic distances. Thirty four alleles were determined, of which nine alleles are novel. The greatest number of alleles was determined in Tarime and Singida white and the lowest in Friesian. Heterozygosities were high within the animals. Phylogenetic tree showed two major clusters one for TSZ and a second for Friesian. Polymorphism at DRB 3.2 in TSZ could be one explanation for their ability to withstand various diseases and we recommend further evaluations in the breed en_US
dc.language.iso en en_US
dc.subject Alleles en_US
dc.subject genetic variation en_US
dc.subject strains en_US
dc.subject Tanzania zebu en_US
dc.title Polymorphisms of BoLA-DRB 3.2 gene and associated genetic relationships among four strains of Tanzania shorthorn zebu cattle en_US
dc.type Article en_US
dc.url https://doi.org/10.12972/jabng.20180039 en_US


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