Sokoine University of Agriculture

Prevalence and characterisation of C TX-M-Type extended spectrum Beta-Lactamase producing Samonella Typhimurium in poultry farms in the Copperbelt province,Zanzibar

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dc.contributor.author Kaonga, Naomi
dc.date.accessioned 2021-05-17T09:20:06Z
dc.date.available 2021-05-17T09:20:06Z
dc.date.issued 2020
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/3531
dc.description Masters Dissertation en_US
dc.description.abstract Poultry is a major reservoir of Salmonella worldwide associated with increasing incidences of strains producing ESBL enzymes that are capable of inactivating a wide variety of β- lactam antibiotics. CTX-M ESBLs have been described in S. Typhimurium isolates with resistance genes located on transferable plasmids. The aim of this study was to determine the prevalence and antimicrobial resistance of S. Typhimurium, prevalence and characterise CTX-M-Type ESBL-producing S. Typhimurium in poultry farms in the Copperbelt Province in Zambia. A cross-section study design was used which involved five districts. One poultry farm per district was randomly selected for sampling of birds. An overall of 384 faecal samples were analysed for the presence of S. Typhimurium using microbiological and molecular methods. S. Typhimurium was detected at 17.7% prevalence in poultry farms of which 12.8% were found harboring the CTX-M-Type ESBL genes. Antibiotic use, purpose of use, withdrawal period, manure handling, hygiene and biosecurity were found to be associated with this prevalence. Chingola district had a prevalence of 7.3% followed by Ndola district with a prevalence of 5.2%, Luanshya district 2.9%, Kitwe 1.6% and Mufulira 0.8%. Further findings indicated that all the isolates showed 100% resistance to tetracycline followed by erythromycin with 97.1%, ampicillin and amoxicillin with 91.2%. A study in Nigeria, reported a prevalence of 16.0% S. Typhimurium in poultry farms and china reported a prevalence on 17.76% CTX-M-Type producing Salmonella in foodborne animals which are slightly similar with findings from this study. Antibiotic resistance to third-generation cephalosporins was at 58.8% cefotaxime and 54.4% ceftazidime. This could be due to the presence of cefotaximases that have more hydrolytic activity to cefotaxime than ceftazidime. en_US
dc.description.sponsorship Inter-University Council for East Africa (IUCEA) en_US
dc.language.iso en en_US
dc.publisher Sokoine University of Agriculture en_US
dc.subject C TX-M-Type en_US
dc.subject Beta-Lactamase en_US
dc.subject Salmonella Typhimurium en_US
dc.title Prevalence and characterisation of C TX-M-Type extended spectrum Beta-Lactamase producing Samonella Typhimurium in poultry farms in the Copperbelt province,Zanzibar en_US
dc.type Thesis en_US


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