Sokoine University of Agriculture

Serological and molecular evidence of brucella species in the rapidly growing pig sector in Kenya

Show simple item record

dc.contributor.author Akoko, J
dc.contributor.author Pelle, R
dc.contributor.author Kivali, V
dc.contributor.author Schelling, E
dc.contributor.author Shirima, G
dc.contributor.author Mathew, C
dc.contributor.author Kyallo, V
dc.contributor.author Bonfoh, B
dc.contributor.author Kazwala, R
dc.contributor.author Ouma, C
dc.contributor.author Machuka, E. M.
dc.contributor.author Fèvre, E. M.
dc.contributor.author Falzon, L. C.
dc.contributor.author Lukambagire, A. S.
dc.contributor.author Halliday, J. E. B.
dc.date.accessioned 2021-05-11T12:51:00Z
dc.date.available 2021-05-11T12:51:00Z
dc.date.issued 2020
dc.identifier.other doi.org/10.1186/s12917-020-02346-y
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/3518
dc.description Journal article en_US
dc.description.abstract Background: Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results: A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RTPCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion: The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs. en_US
dc.description.sponsorship supported within the framework of the DELTAS Africa Initiative [Afrique One-ASPIRE /DEL-15-008]. The Medical Research Council (UK) supported data collection and serological analysis, Biotechnology and Biological Science Research Council (UK), the Economic and Social Research Council (UK), the Natural Environment Research Council (UK), through the Environmental & Social Ecology of Human Infectious Diseases Initiative (ESEI), Grant Reference: G1100783/1 and the CGIAR Research Program on Agriculture for Nutrition and Health (A4NH), led by the International Food Policy Research Institute (IFPRI); we acknowledge the CGIAR Fund Donors (https:// www.cgiar.org/funders/). The molecular assays were conducted at the BecAILRI Hub through the Africa Biosciences Challenge Fund (ABCF) fellowship. The ABCF program is funded by the Australian Department for Foreign Affairs and Trade (DFAT) through the BecA-CSIRO partnership; the Syngenta Foundation for Sustainable Agriculture (SFSA); the Bill & Melinda Gates Foundation (BMGF); the UK Department for International Development (DFID) and the Swedish International Development Co-operation Agency (Sida). en_US
dc.language.iso en en_US
dc.publisher BMC Veterinary Research en_US
dc.subject Pig brucellosis en_US
dc.subject Molecular detection en_US
dc.subject Molecular evidence en_US
dc.subject Brucella en_US
dc.subject Serology en_US
dc.subject Kenya en_US
dc.title Serological and molecular evidence of brucella species in the rapidly growing pig sector in Kenya en_US
dc.type Article en_US
dc.url https://pubmed.ncbi.nlm.nih.gov/32393374/ en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search SUA IR


Browse

My Account