Sokoine University of Agriculture

Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms

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dc.contributor.author Mgode, G.F
dc.contributor.author Weetjens, B
dc.contributor.author Cox, C
dc.contributor.author Jubitana, M
dc.contributor.author Machang’u, R
dc.contributor.author Lazar, D
dc.contributor.author Weiner, J
dc.contributor.author Geertruyden, J.P
dc.contributor.author Kaufmann, S
dc.date.accessioned 2017-03-14T11:28:48Z
dc.date.available 2017-03-14T11:28:48Z
dc.date.issued 2011-11-26
dc.identifier.uri http://www.suaire.sua.ac.tz/handle/123456789/1339
dc.description Tuberculosis 2011, vol 87:182 -186 en_US
dc.description.abstract Trained African giant pouched rats (Cricetomys gambianus) have potential for diagnosis of tuberculosis (TB). These rats target volatile compounds of Mycobacterium tuberculosis (Mtb) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus spp., which are also acid-fast bacilli and can be misdiagnosed as Mtb in smear microscopy. Diagnostic performance of C. gambianus on in vitro-cultured mycobacterial and related pulmonary microbes is unknown. This study reports on the response of TB detection rats to cultures of reference Mtb, clinical Mtb, NTM, Nocardia; Rhodococcus; Streptomyces; Bacillus; and yeasts. Trained rats significantly discriminated Mtb from other microbes (p < 0.008, Fisher’s exact test). Detection of Mtb cultures was age-related, with exponential and early stationary phase detected more frequently than early log phase and late stationary phase (p < 0.001, Fisher’s test) (sensitivity ¼ 83.33%, specificity ¼ 94.4%, accuracy ¼ 94%). The detection of naturally TB-infected sputum exceeded that of negative sputum mixed with Mtb, indicating that C. gambianus are conditioned to detect odours of TB-positive sputum better than spiked sputum. Although further studies on volatiles from detectable growth phases of Mtb are vital for identification of Mtb-specific volatiles detected by rats, our study underline the potential of C. gambianus for TB diagnosis. en_US
dc.language.iso en en_US
dc.subject Tuberculosis diagnosis en_US
dc.subject Nontuberculous mycobacteria en_US
dc.subject Growth phase en_US
dc.subject Volatile compounds en_US
dc.title Ability of Cricetomys rats to detect Mycobacterium tuberculosis and discriminate it from other microorganisms en_US
dc.type Article en_US
dc.url http://intl.elsevierhealth.com/journals/tube en_US


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